Containing plasma around the LDH activity inside the myocardial ?cell culture supernatant and Na+-K+- ATPase and Ca2+- ATPase activity in the myocardial cell (X ?s)Experimental group Blank manage group Injured group High-concentration ferulic acid group Medium-concentration ferulic acid group Low-concentration ferulic acid group Drug-containing plasma containing high concentrations of ferulic acid Drug-containing plasma containing medium Concentrations of ferulic acid Drug-containing plasma containing low concentrations of ferulic acidNote: Compare to regular handle group, *P 0.LDH (U/L) 68.7 ?two.31* 218 ?7.13* 144 ?12.8* 172 ?five.21* 186 ?7.13* 128 ?3.92* 141 ?four.1* 113 ?eight.38*Na+-K+- ATPase (umol.mg-.h-) 0.581 ?0.212* 0.478 ?0.077* 0.552 ?0.213* 0.514 ?0.032* 0.503 ?0.102* 0.562 ?0.411* 0.53 ?0.159* 0.571 ?0.613*Ca2+- ATPase (umol.mg-.h-) 0.791 ?0.125* 0.455 ?0.137* 0.613 ?0.121* 0.57 ?0.03* 0.541 ?0.031* 0.654 ?0.11* 0.611 ?0.024* 0.681 ?0.159*Pharmacognosy Magazine | July-September 2013 | Vol 9 | IssueRen, et al.: Protective effects of ferulic acid on key cultured neonatal rat cardiomyocytesTable six: Outcome of effects of ferulic acid and its drug-containing plasma on spontaneous ?frequency of myocardial cell (X ?s)Groups Just before Right after 1 two 45 ?14 0 min 73 ?14* 78 ?13* 5 min 75 ?12* 79 ?18* 10 min 77 ?11* 81 ?15* Beating price 20 min 79 ?10* 82 ?16* 30 min 80 ?11* 83 ?10*Note: 1 = the optimal concentration of ferulic acid, two = the optimal concentration of ferulic acid containing plasma, Examine to typical control group, *P 0.Table 7: Outcome of effects of ferulic acid and its drug-containing plasma on possible across ?plasma membrane of myocardial cells (X ?s)Time/ min Ahead of Just after 0 min 0 min 5 min 5 min 10 min 10 min 20 min 20 min 30 min 30 min 1 two 1 2 1 two 1 2 1 two MDP (-mv) 68.7 ?5.7* 65.4 ?1.91* 66.7 ?five.2* 65.6 ?2.1* 66.9 ?7.7* 65.9 ?2.3* 67.3 ?three.82* 67.2 ?six.3* 69.0 ?5.8* 66.four ?five.23* 68.3 ?four.8* APA (mv) 72.5 ?4.72* 61.4 ?two.1* 62.5 ?3.2* 63.1 ?4.6* 63.9 ?six.0* 63.six ?4.8* 64.four ?four.1* 65.0 ?two.3* 66.4 ?3.23* 64.7 ?three.6* 65.8 ?three.3* Vmax (v/s) 24 ?4.1* 19.2 ?three.3* 20.6 ?three.2* 21.4 ?six.1* 22.8 ?five.3* 20.five ?five.8* 23.4 ?4.9* 21.6 ?5.91* 23.six ?5.248274-16-0 Purity 1* 20.6 ?five.6* 21.five ?3.2*of instruction bottle and continue which referred to as hypoxia 1.TCEP (hydrochloride) custom synthesis five h for oxygen, then to oxygen for 1 h, with typical medium nitrogen to replace the saturated medium, and continue to culture for 1 h, prepare the experiment model of hypoxia/reoxygenation.PMID:32926338 But for the duration of this system, nitrogen is easy to the loss and just isn’t straightforward to control the content of nitrogen, and repeatability of operation is poorer. Zeng Jing etc[27] transform for no sugar serumfree media, drop two mL sterilization liquid paraffin wax into each effectively of education board, and closed for two, four, 8, 12 h, respectively, suck out paraffin wax, and wash with sterilizated of PBS for three occasions. After which change for the serum containing 15 DMEM medium, and continue to culture for 2 h in CO2 incubator, that is reoxygenation. However the liquid paraffin wax might have specific effect to the development of cells. And have bad effect to the accuracy of experimental final results, Consequently, in this experiment we chose Na2S204 to lead to the damage of myocardial cells, Na2S204, as an oxygen remover, doesn’t injure cell membrane, and commonly appropriate dose can take away oxygen in culture medium can cause cardiomyocyte hypoxia; replacing medium soon after hypoxia results in reoxygenation. This method is basic and quick to operate, and has superior repeatability, is regarded as as an excellent meth.